CIA 膠原誘導(dǎo)的關(guān)節(jié)炎模型
更新時(shí)間：2012-08-31 點(diǎn)擊次數(shù)：7358
膠原誘導(dǎo)的關(guān)節(jié)炎模型 （CIA）
實(shí)驗(yàn)方案
1. 牛II型膠原溶解在0.05mol/ml的乙酸中，濃度為2mg/ml，攪拌4°C過(guò)夜。
2. 注射前，II膠原溶液與同體積的IFA在冰浴中*乳化。
3. *天，每只大鼠尾根部皮內(nèi)注射0.1ml CII/IFA乳化劑（濃度為1mg/ml）。
4. 第7天，每只大鼠以同樣的方式再次注射。
5. 注射后，大鼠關(guān)節(jié)炎指數(shù)根據(jù)肉眼評(píng)分系統(tǒng)每周兩次進(jìn)行評(píng)價(jià)。
0－正常
1－一個(gè)足爪腫脹和/或發(fā)紅
2－2個(gè)相關(guān)關(guān)節(jié)腫脹和/或發(fā)紅
3－>2個(gè)相關(guān)關(guān)節(jié)腫脹和/或發(fā)紅
4－整個(gè)足爪嚴(yán)重炎癥
每只大小鼠關(guān)節(jié)炎指數(shù)為每只動(dòng)物4足爪得分之和。
6. 腫脹嚴(yán)重性根據(jù)器官充滿(mǎn)度測(cè)定法通過(guò)氣道容積的改變來(lái)測(cè)定， 一周兩次。
Brief protocol of Collagen induced arthritis model （CIA）
1. Bovine CII is dissolved in 0.05mol/L acetic acid at a concentration of 2 mg/ml by stirring overnight at 4°C.
2. Before injection, CII is emulsified with an equal volume of IFA in an ice-cold water bath.
3. On day1, each rat is injected intradermally at the base of the tail with 0.1ml CII/IFA the emulsion （1mg/ml）.
4. On day 21, a second injection of CII in IFA will be administered using the same amount.
5. After the second injection，rats will be evaluated daily for arthritis using a macroscopic scoring system of 0–4.
0 no signs of arthritis
1 swelling and/or redness of the paw or 1 digit
2=2 joint involved
3=>2 joint involved
4severe arthritis of the entire paw and digits
An arthritis index for each rat is calculated by adding the scores for each of the 4 paws.
6. Swelling severity is also determined by quantitating the change in the paw volume, as measured by plethysmometry.
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